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Registro Completo |
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Biblioteca(s): |
Biblioteca Rui Tendinha. |
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Data corrente: |
08/01/2014 |
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Data da última atualização: |
01/09/2015 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ABREU, P. M. V.; PICCIN, J. G.; RODRIGUES, S. P.; VENTURA, J. A.; FERNANDES, P. M. B. |
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Afiliação: |
Paolla M.V. Abreu, UFES; João G. Piccin, UFES; Silas P. Rodrigues, UFES; Jose Aires Ventura, Incaper; Patricia M.B. Fernandes, UFES. |
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Título: |
Molecular diagnosis of Papaya meleira virus (PMeV) from leaf samples of Carica papaya L. using conventional and real-time RT-PCR. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Journal of Virological Methods, Amsterdam-Holanda, v. 180, p. 11-17, 2012. |
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Páginas: |
7p. |
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ISSN: |
0166-0934 |
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DOI: |
10.1016/j.jviromet.2011.12.003 |
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Idioma: |
Português |
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Conteúdo: |
Papaya meleira virus (PMeV) is the causal agent of papaya sticky disease. This study describes two methods for molecular diagnosis of PMeV using conventional and real-time PCR. These methods were shown to be more efficient than current methods of viral detection using extraction of PMeV dsRNA and observation of symptoms in the field. The methods described here were used to evaluate the effect of inoculation of papaya plants with purified PMeV dsRNA on the progress of PMeV infection. A single inoculation with PMeV dsRNA was observed to delay the progress of the virus infection by several weeks. The possibility of vertical transmission of PMeV was also investigated. No evidence was found for PMeV transmission through seeds collected from diseased fruit. The implications of these results for the epidemiology of PMeV and the management of papaya sticky disease are discussed. |
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Palavras-Chave: |
DsRNA; Papaya; Papaya meleira virus; Phytopathology; Real-time RT-PCR; Resistance induction. |
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Categoria do assunto: |
-- |
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URL: |
http://ac.els-cdn.com/S0166093411004885/1-s2.0-S0166093411004885-main.pdf?_tid=597935f2-7857-11e3-a060-00000aacb35e&acdnat=1389180411_31ada3a608c8d17c0a26fd345cb062ae
http://biblioteca.incaper.es.gov.br/digital/bitstream/item/162/1/JVirolMethods-2012.PDF
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Marc: |
LEADER 01746naa a2200277 a 4500
001 1001438
005 2015-09-01
008 2012 bl --- 0-- u #d
022 $a0166-0934
024 7 $a10.1016/j.jviromet.2011.12.003$2DOI
100 1 $aABREU, P. M. V.
245 $aMolecular diagnosis of Papaya meleira virus (PMeV) from leaf samples of Carica papaya L. using conventional and real-time RT-PCR.$h[electronic resource]
260 $c2012
300 $a7p.
520 $aPapaya meleira virus (PMeV) is the causal agent of papaya sticky disease. This study describes two methods for molecular diagnosis of PMeV using conventional and real-time PCR. These methods were shown to be more efficient than current methods of viral detection using extraction of PMeV dsRNA and observation of symptoms in the field. The methods described here were used to evaluate the effect of inoculation of papaya plants with purified PMeV dsRNA on the progress of PMeV infection. A single inoculation with PMeV dsRNA was observed to delay the progress of the virus infection by several weeks. The possibility of vertical transmission of PMeV was also investigated. No evidence was found for PMeV transmission through seeds collected from diseased fruit. The implications of these results for the epidemiology of PMeV and the management of papaya sticky disease are discussed.
653 $aDsRNA
653 $aPapaya
653 $aPapaya meleira virus
653 $aPhytopathology
653 $aReal-time RT-PCR
653 $aResistance induction
700 1 $aPICCIN, J. G.
700 1 $aRODRIGUES, S. P.
700 1 $aVENTURA, J. A.
700 1 $aFERNANDES, P. M. B.
773 $tJournal of Virological Methods, Amsterdam-Holanda$gv. 180, p. 11-17, 2012.
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Registro original: |
Biblioteca Rui Tendinha (BRT) |
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Registro Completo |
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Biblioteca(s): |
Biblioteca Rui Tendinha. |
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Data corrente: |
24/11/2020 |
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Data da última atualização: |
30/11/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
JÚNIOR, A. L. da S.; CABRAL, R. L. R.; SARTORI, L.; SOUZA, L. C. de; MIRANDA, F. D. de; CALDEIRA, M. V. W.; MOREIRA, S. O.; GODINHO, T. de O. |
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Afiliação: |
Adelson Lemes da Silva Júnior, UFES; Rafael Lara Rezende Cabral, UFES; Lara Sartori, UFES; Lucimara Cruz de Souza, UFES; Fábio Demolinari de Miranda, UFES; Marcos Vinicius Winckler Caldeira, UFES; Sarah Ola Moreira, Incaper; Tiago de Oliveira Godinho, Vale Linhares. |
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Título: |
Evaluation of the diversity and genetic struture as strategies for conservation of natural populations of Dalbergia nigra (Vell.) Allemão ex Benth. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
CERNE, v. 26, n. 4, p. 435-443, nov. 2020. |
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Idioma: |
Inglês |
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Conteúdo: |
The evaluation of diversity and genetic structure allows us to verify with precision the effect of evolutionary and anthropic processes on species. The objective of this research was to evaluate the divergence and the genetic structure of two natural populations of Dalbergia nigra, using molecular markers Inter Simple Sequence Repeats (ISSR). Leaf samples were collected from two populations, located in the National Forest of Pacotuba and the Private Natural Heritage Reserve of Cafundó. Eight ISSR primers were used, which resulted in 97 bands, with 68.04% of polymorphism. Based on the joint data, the values of 0.33 for the Nei index (H*) and 0.50 for the Shannon index (I*) indicated moderate to high genetic diversity, being influenced by the presence of genetically dissimilar individuals in the National Forest of Pacotuba. Most of the genetic divergence was intrapopulational (85.96%), with moderate differentiation between populations (ΦST = 0.1404). The estimated historical gene flow between the fragments was low (Nm = 3.21) when compared with results from other tree species, and the genetic structuring analysis separated the populations into two groups, corresponding to the two populations evaluated. The results indicate a small genetic share among populations, however, populations are becoming structured. The satisfactory levels of genetic diversity benefit the use of the trees as matrixes for programs of restoration and recovery of degraded areas, connectivity of landscapes, and sustainable use of forest resources. MenosThe evaluation of diversity and genetic structure allows us to verify with precision the effect of evolutionary and anthropic processes on species. The objective of this research was to evaluate the divergence and the genetic structure of two natural populations of Dalbergia nigra, using molecular markers Inter Simple Sequence Repeats (ISSR). Leaf samples were collected from two populations, located in the National Forest of Pacotuba and the Private Natural Heritage Reserve of Cafundó. Eight ISSR primers were used, which resulted in 97 bands, with 68.04% of polymorphism. Based on the joint data, the values of 0.33 for the Nei index (H*) and 0.50 for the Shannon index (I*) indicated moderate to high genetic diversity, being influenced by the presence of genetically dissimilar individuals in the National Forest of Pacotuba. Most of the genetic divergence was intrapopulational (85.96%), with moderate differentiation between populations (ΦST = 0.1404). The estimated historical gene flow between the fragments was low (Nm = 3.21) when compared with results from other tree species, and the genetic structuring analysis separated the populations into two groups, corresponding to the two populations evaluated. The results indicate a small genetic share among populations, however, populations are becoming structured. The satisfactory levels of genetic diversity benefit the use of the trees as matrixes for programs of restoratio... Mostrar Tudo |
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Palavras-Chave: |
Jacarandá-da-baía. |
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Thesagro: |
Dalbergia Nigra; Genética Vegetal. |
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Thesaurus NAL: |
Ecological genetics. |
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Categoria do assunto: |
K Ciência Florestal e Produtos de Origem Vegetal |
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URL: |
https://biblioteca.incaper.es.gov.br/digital/bitstream/123456789/4124/1/dalbergia-nigra-moreira.pdf
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Marc: |
LEADER 02411naa a2200253 a 4500
001 1022978
005 2020-11-30
008 2020 bl uuuu u00u1 u #d
100 1 $aJÚNIOR, A. L. da S.
245 $aEvaluation of the diversity and genetic struture as strategies for conservation of natural populations of Dalbergia nigra (Vell.) Allemão ex Benth.$h[electronic resource]
260 $c2020
520 $aThe evaluation of diversity and genetic structure allows us to verify with precision the effect of evolutionary and anthropic processes on species. The objective of this research was to evaluate the divergence and the genetic structure of two natural populations of Dalbergia nigra, using molecular markers Inter Simple Sequence Repeats (ISSR). Leaf samples were collected from two populations, located in the National Forest of Pacotuba and the Private Natural Heritage Reserve of Cafundó. Eight ISSR primers were used, which resulted in 97 bands, with 68.04% of polymorphism. Based on the joint data, the values of 0.33 for the Nei index (H*) and 0.50 for the Shannon index (I*) indicated moderate to high genetic diversity, being influenced by the presence of genetically dissimilar individuals in the National Forest of Pacotuba. Most of the genetic divergence was intrapopulational (85.96%), with moderate differentiation between populations (ΦST = 0.1404). The estimated historical gene flow between the fragments was low (Nm = 3.21) when compared with results from other tree species, and the genetic structuring analysis separated the populations into two groups, corresponding to the two populations evaluated. The results indicate a small genetic share among populations, however, populations are becoming structured. The satisfactory levels of genetic diversity benefit the use of the trees as matrixes for programs of restoration and recovery of degraded areas, connectivity of landscapes, and sustainable use of forest resources.
650 $aEcological genetics
650 $aDalbergia Nigra
650 $aGenética Vegetal
653 $aJacarandá-da-baía
700 1 $aCABRAL, R. L. R.
700 1 $aSARTORI, L.
700 1 $aSOUZA, L. C. de
700 1 $aMIRANDA, F. D. de
700 1 $aCALDEIRA, M. V. W.
700 1 $aMOREIRA, S. O.
700 1 $aGODINHO, T. de O.
773 $tCERNE$gv. 26, n. 4, p. 435-443, nov. 2020.
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